Method IV

Plate streaking with inoculum from another plate:

Repeat the steps of plate streaking from method III, except that the inoculum is taken from another Petri dish.

Find a single colony on the original agar plate that is located nearby Line D and is physically isolated from all other colonies on the same plate. This colony is homogeneous in the sense that all the cells degenerate from the same parent. Thus, this can be considered as a pure culture.

Flame the loop and lift a minute amount of culture from the original plate. Remember that one need not see the colony of organisms on his loop to prove that that the microorganisms are indeed there. There is already too much if one can see the culture on the inoculation loop.

Transfer the cells in the loop to a new agar plate by following the same set of steps involved in streaking a plate as before. Incubate the inoculated plate.

Yeast Culturing Page