After autoclaving the media for 20 minutes, the tubes are placed in a slanted position to allow the agar to solidify. These tubes are called slants.
Method II
Slant tube preparation:
Mix and prepare the YPG nutrient agar as in the preparation of Petri dishes. Pour 6 ml of the nutrient into a test tube. Plug the test tube with a cotton ball or a cap. Culture tubes with screw caps may also be used.
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